Reporter

Part:BBa_K1179018:Design

Designed by: Brandon Nadres   Group: iGEM13_MIT   (2013-09-15)


Fluorescent mKate protein with a fused PEST site for rapid degradation


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 875
    Illegal XbaI site found at 871
    Illegal PstI site found at 825
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 875
    Illegal PstI site found at 825
    Illegal NotI site found at 860
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 875
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 875
    Illegal XbaI site found at 871
    Illegal PstI site found at 825
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 875
    Illegal XbaI site found at 871
    Illegal PstI site found at 825
    Illegal NgoMIV site found at 751
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 656
    Illegal SapI.rc site found at 38


Design Notes

Not applicable.

Source

Sincere thanks to Samira Kiani for contributing this entry vector to the 2013 MIT iGEM team.

References